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The cells should not be old, preferably 1st to 4th passage is good for high transfection efficiency. Also you should increase the DNA amount. I use 10 ug DNA for 100 mm culture plates and 3 ug for

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The Reagent Distributor is an accessory machine to the Wet Reagent Feeder. It accurately distributes the reagents from the feeder to every cell in the flotation circuit in precisely the proper quantities to give the highest efficiency possible.

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